Put Through __TOP__
Planning ahead and packing properly can facilitate the screening process and ease your travel experience at the airport. Know what you can pack in your carry-on and checked baggage before arriving at the airport by reviewing the lists below. Even if an item is generally permitted, it may be subject to additional screening or not allowed through the checkpoint if it triggers an alarm during the screening process, appears to have been tampered with, or poses other security concerns. Read about civil penalties for prohibited items.
Anthrax has been used as a weapon around the world for nearly a century. In 2001, powdered anthrax spores were deliberately put into letters that were mailed through the U.S. postal system. Twenty-two people, including 12 mail handlers, got anthrax, and five of these 22 people died.
Overall, 56% of all TF antigens screened generated Fabs that passed primary and secondary validation criteria. We believed some of this failure could be from inefficient protein folding in E. coli. Therefore, we sought to develop additional high through-put antigen expression systems to supplement E. coli expression system. To systematically compare expression methods and selections from them, we studied the ability to produce Fabs from 32 different TF antigens expressed in three different formats: E. coli, in vitro transcription translation, and yeast antigen display.
Laboratory automation was critical to standardize the many routine and laborious activities ranging from phage sorting, ELISA, colony picking, PCR and stability measurements to facilitate large-scale generation of affinity reagents. The automated selection process greatly reduced the time from months in animals to weeks on the robot with reduced costs associated with antibody generation, and increased the throughput to up to 48 per batch of antibody selections. To take maximal advantage of the robotics it was necessary to make numerous adjustments in the bench scale phage and antigen protocols. For example, the automated magnetic bead selections coupled with the catch and release approach permitted clean elution of antigen-bound phage of very high affinity that can be problematic for low pH glycine elution. Moreover, protocols for preclearing the starting library and enriching selection pools for functional phage using Protein A purification have been added.
We attribute much of the success here to choosing a remarkably stable Fab library scaffold (average Tm 80 C). The selected Fabs show consistently high expression by simple shake flask culturing in E. coli. They are easily purified in high-throughput using simple Protein A columns. This synthetic library built on a highly stable and well-expressed scaffold can provide more consistent stability and expression than for natural B-cell derived libraries (7), which have multiple scaffolds that vary widely in stability and recombinant expression.
The recombinant antibodies have characteristics of highly selective binding reagents based on the high throughput validation data presented: stringent selection by phage display, unique sequences for each Fab, high stability and affinity, high and soluble expression in E. coli, competitive binding with antigen, binding in the presence of complex cell lysates, and specific immunofluorescence binding patterns in cells. Although no systematic analysis of the suitability of the antibodies for Western blotting has been done, we do not anticipate high performance because the Fabs were intentionally raised to soluble protein domains. Nonetheless, for about 20 Fabs tested with collaborators, five showed a Western positive band corresponding to the TF suggesting a subset will work in Western blots (Gordon Whiteley, National Cancer Institute, unpublished results).
Bronchoscopy is a procedure that allows a health care provider to look at your lungs. It uses a thin, lighted tube called a bronchoscope. The tube is put through the mouth or nose and moved down the throat and into the airways. It helps diagnose and treat certain lung diseases.
Bronchoalveolar lavage (BAL) is a procedure that is sometimes done during a bronchoscopy. It is also called bronchoalveolar washing. BAL is used to collect a sample from the lungs for testing. During the procedure, a saline solution is put through the bronchoscope to wash the airways and capture a fluid sample.
If scar tissue does form inside the stent, you may need a repeat procedure. This may be using either balloon angioplasty or with a second stent. In some cases, radiation therapy may be given through a catheter placed near the scar tissue to stop the growth of scar tissue and open up the vessel. This is called brachytherapy.
There will be several monitor screens in the room, showing your vital signs, the images of the catheter being moved through your body into your heart, and the structures of your heart as the dye is injected.
Once the local anesthesia has taken effect, a sheath, or introducer, will be put into the blood vessel (often at the groin). This is a plastic tube through which the catheter will be threaded into the blood vessel and advanced into the heart.
The catheter will be threaded through the sheath into the blood vessel. The doctor will advance the catheter through the aorta into the heart. Fluoroscopy will be used to help see the catheter advance into the heart.
The catheter will be threaded into the coronary arteries. Once the catheter is in place, contrast dye will be injected through the catheter into your coronary arteries in order to see the narrowed area(s). You may feel some effects when the contrast dye is injected into the IV line. These effects include a flushing sensation, a salty or metallic taste in the mouth, or a brief headache. These effects usually last only a few moments.
put through the wringer (third-person singular simple present puts through the wringer, present participle putting through the wringer, simple past and past participle put through the wringer)
People with cancer often get treatment through a vein. The medical term for this is intravenous (IV) treatment. Your health care team can give you chemotherapy, other drugs, blood transfusions, and fluids this way. Medical devices called catheters and ports make intravenous treatments easier. They can also make it easier for your health care team to take blood samples.
IV catheter. Treatments that are given in a vein are often given through a catheter with a small needle. This is called an IV catheter or simply an "IV." Your nurse puts the needle in your forearm or the top of your hand. Besides chemotherapy, you can get other drugs this way. For example, you might get medication that helps with nausea in your IV.
Implantable port or port-a-cath. A surgeon or radiologist puts in a port. This is usually done with local anesthesia or conscious sedation. The entire catheter goes under the skin of your chest or upper arm. To give treatment with a port, your nurse may first numb the skin with cream. Then, your nurse cleans the skin and puts a needle into the port. Treatment or blood samples go through the needle.
Let you have some chemotherapy at home instead of the hospital or clinic, for certain treatment plans. You can get continuous infusion therapy this way. During this type of therapy, you receive treatment drugs through a small pump that you wear or carry.
Insights from appraisal theories of emotion are used to integrate elements of theories on collective action. Three experiments with disadvantaged groups systematically manipulated procedural fairness (Study 1), emotional social support (Study 2), and instrumental social support (Study 3) to examine their effects on collective action tendencies through group-based anger and group efficacy. Results of structural equation modeling showed that procedural fairness and emotional social support affected the group-based anger pathway (reflecting emotion-focused coping), whereas instrumental social support affected the group efficacy pathway (reflecting problem-focused coping), constituting 2 distinct pathways to collective action tendencies. Analyses of the means suggest that collective action tendencies become stronger the more fellow group members "put their money where their mouth is." The authors discuss how their dual pathway model integrates and extends elements of current approaches to collective action.
"We've all kind of gone through our trial run using this vest," Coffield said. "The side plates they issued us are a little smaller and a little lighter. They definitely fit a lot better as far as sitting on our hips."
Provide emergency paid leave to 106 million more Americans to reduce the spread of the virus. No American should have to choose between putting food on the table and quarantining to prevent further spread of COVID-19. And yet, nearly 1 in 4 workers and close to half of low-income workers lack access to paid sick leave, disproportionately burdening Americans of color. Lack of paid leave is threatening the financial security of working families and increasing the risk of COVID-19 infections, hospitalizations, and deaths. Congress did the right thing last year when it created an emergency paid leave program through the Families First Coronavirus Response Act. That action decreased daily infections by 400 cases per state per day in states that previously had no paid sick leave requirement. While the December down payment extended the Families First employer tax credits through March 2021, it did not renew the requirement that employers provide leave. President Biden is calling on Congress to:
As a result of the COVID-19 crisis, millions of Americans are hurting through no fault of their own. More than 10 million Americans are unemployed, and 4 million have been out of work for half a year or longer. The jobs crisis is particularly severe in communities of color, where 1 in 10 Black workers and 1 in 11 Latino workers are unemployed. Large numbers of families are struggling to pay rent or their mortgages and put food on the table. And, last month, it only got worse: we lost 140,000 jobs in December, including 20,000 public educators, and nearly 400,000 jobs at restaurants and bars. 041b061a72