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Logan Adams
Logan Adams

Manual Of Commercial Methods In Clinical Microbiology


The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition presents a wealth of relevant information to clinical pathologists, directors and supervisors of clinical microbiology, infectious disease physicians, point-of-care laboratories, professionals using industrial applications of diagnostic microbiology and other healthcare providers. The content will allow professionals to analyze all commercially available methods to determine which works best in their particular laboratory, hospital, clinic, or setting.




manual of commercial methods in clinical microbiology



"The second edition of the Manual of Commercial Methods in Clinical Microbiology: International Edition is intended to be a current reference of commercially available tests, devices, and instruments used in clinical microbiology. The first edition contained 18 chapters that focused on US regulatory issues, rapid detection systems for bacteria and viruses, blood culture systems, diagnostic parasitology, automated serology, susceptibility testing, molecular methods, laboratory information systems, and specific pathogens such as HIV, Chlamydia, Mycoplasma, Mycobacterium, and emerging organisms. The second edition has been greatly updated and expanded to 35 chapters including new chapters on virology, viral hepatitis, automated molecular typing systems, specific pathogens such as human papillomavirus and rickettsiae, tickborne diseases, and bioterrorism. Additionally, there are 11 new chapters containing a synopsis of international clinical microbiology products and the regulatory requirements of selected countries including several in Europe and Asia, as well as Canada, Australia, South Africa, and Argentina.


The content of this book focuses primarily on human clinical microbiology; however, both the first and second editions include a chapter on Commercial Methods in Clinical Veterinary Microbiology coauthored by microbiologists from 3 colleges of veterinary medicine. That chapter is an informed summary that reveals issues associated with the use of human diagnostic systems in veterinary microbiology and includes references that document the accuracy of such systems when used to analyze samples of various animal origins....


Although the majority of the book focuses on available tests for human pathogens, a reasonable amount of the reference information will be useful for veterinary diagnostic microbiologists. The inclusion of the chapter on veterinary clinical veterinary microbiology integrates the reference material in an informative manner. It is a comprehensive reference of available commercial diagnostic systems for laboratory managers seeking tools for enhancing efficiency and accuracy." (Journal of the American Veterinary Medical Association, 15th Dec 2017)


FDA's Bacteriological Analytical Manual (BAM) presents the agency's preferred laboratory procedures for microbiological analyses of foods and cosmetics. AOAC International published previous editions of this manual in a loose-leaf notebook format, and, more recently, on CD-ROM. This online BAM is now available to the public. Some changes have been made to methods since the previous version. A listing of chapters updated since the last hard-copy version (Edition 8, Revision A /1998) can be found in About the Bacteriological Analytical Manual. The members of the BAM Council are listed below. In addition recent changes for most Chapters are documented in a brief Revision History at the beginning of the Method. There is also e-mail contact information for each Chapter. Chapter numbers have been retained from the previous version. However, for this Table of Contents, chapters have been grouped by category. Please send comments to Karen Jinneman.


Innovations in methods for the microbiological analysis of food continue to appear at a rapid pace. Edition 8 (1995) of the Bacteriological Analytical Manual (BAM-8) contained numerous refinements of procedures and updates of references from the 1992 edition. The list of commercially available test kits and the discussion of rapid methods in Appendix 1 were thoroughly revised. Three chapters were added: the use of reverse transcription (RT) and the polymerase chain reaction (PCR) to detect and quantify contamination of shellfish with hepatitis A virus (Chapter 26); new procedures for the alkaline phosphatase test to determine whether dairy foods were prepared with pasteurized milk (Chapter 27); and the use of PCR to detect toxigenic Vibrio cholerae in foods (Chapter 28). For this printing (BAM - 8A), the following has been revised or added: Campylobacter (Chapter 7), Yeast and Molds (Chapter 18), Cyclospora [Chapter 19 (Parasites)] and Staphylococcus enterotoxins (Chapter13). In addition, there are updated tables in Appendix 1 on Rapid Methods and revised and corrected tables in Appendix 2 on MPN. Appendix 3 reflects changes in media and corrects errors in the 8th Edition. A table summarizing changes from BAM-8 to BAM-8A is included.


The methods described in Chapters 1 to 28 are those preferred by FDA for the microbiological analysis of foods, drinks, and cosmetics as well as for their containers, contact materials, and the production environment. This is not necessarily the case for the rapid methods listed in Appendix 1: this appendix is a listing of different kits that are commercially available. These methods have not necessarily been evaluated by FDA, and listing of a method in this appendix does not constitute a recommendation.


The ability of microorganisms to grow and reproduce in cosmetic products has been known for many years. Microorganisms may cause spoilage or chemical changes in cosmetic products and injury to the user (4,5,10,14-16,20,21). Methods for isolation of microorganisms from cosmetic products are direct colony counts and enrichment culturing. Products that are not soluble in water are initially treated to render them miscible before isolation procedures are conducted. Dilution and plating media that partially inactivate preservative systems commonly found in cosmetic products are used. The isolated microorganisms are identified by routine microbiological methods or by commercial identification kits.


Learn more about the latest clinical microbiology issues and trends from experts working in the field, and discover advances in blood culture, susceptibility testing and other microbiology workflow solutions.


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